Anti-HepA [HA1] Ab00920-23.0 from Absolute Antibody

Product Specs
ItemAnti-HepA [HA1]
CompanyAbsolute Antibody
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAb00920-23.0
Quantity200 µg
ApplicationsRadioimmunofocus inhibition assays, ELISA
Conjugate/TagUnconjugated, AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
TargetHepA
IsotypeIgG
CloneHA1
HostRabbit
ImmunogenGenerated mAbs to HAV by phage display of the antibody library generated from the peripheral blood lymphocytes of immune donor recovered from hepatitis A. The cDNAs encoding the immunoglobulin gamma1 Fd and kappa light chains were synthesized from total RNA of the PBLs of the HAV-immune donors and cloned into a phage display vector to construct a combinatorial library of clones. The phage-displayed Fab library was panned four times against HAV. Analysis of the antigen-binding activities of the pooled phages after each round of the panning indicated that HAV-binding phage clones were almost selected after the third round of panning. Finally, 24 phage clones after the fourth panning were selected, and the gene III fragment was removed to produce soluble Fab. Analysis of the antigen-binding activities of the 24 Fab clones by ELISA revealed that 22 clones were positive in HAV binding, which could be grouped into 4 different clones; HA1, HA6, HA9, and HA12
ClonalityMonoclonal
ReactivityHepatitis Virus
Research AreaVirology; Immunology
PurityProtein A affinity purified